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Just how do i Don Ugg boot Blow Momma Footwear?

Author: zadaw

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February 29th, 2012  |  Posted in Health Care & Medical  |  No Comments »

How do you Wear Ugg sheepskin boots Filler Momma Shoes or boots?

Author: zadaw

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February 27th, 2012  |  Posted in Health Care & Medical  |  No Comments »

Low priced Ugg boot : Ugg boot Electric outlet : How can i See them

Author: zadaw

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February 8th, 2012  |  Posted in Health Care & Medical  |  No Comments »

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Author: zadaw

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February 6th, 2012  |  Posted in Arts & Entertainment  |  No Comments »

Serological Tests for HIV Infection

Author: admin

1. Serology - the diagnosis of HIV infection is usually based on serological tests.

(a) Antibody tests – ELISAs are the most frequently used method for screening of blood samples for HIV antibody. The sensitivity and specificity of the presently available commercial systems approaches 100% but false positive and false negative reactions occur. Other test systems available include passive particle agglutination, immunofluorescence, Western blots and RIPA bioassays. Western blots are regarded as the gold standard and seropositivity is diagnosed when antibodies against both the env and the gag proteins are detected. The sensitivity of the test systems are currently being improved by the use of recombinant antigens.

Microplate ELISA: coloured wells indicate reactivity

Interpretation of Western blot results for HIV antibody

October 1st, 2011  |  Posted in Diagnosis of HIV  |  No Comments »

Monitoring Anti-HIV therapy

Author: admin

a. Viral Load

  1. Initiation – viral load is now the preferred method of monitoring therapy. There should be >= 1 log reduction in viral load, preferably to less than 10,000 copies/ml HIV-RNA within 2-4 weeks after the commencement of treatment. If <0.5 log reduction in viral, or HIV-RNA stays above 100,000, then the treatment should be adjusted by either adding or switching drugs.
  2. Monitoring – viral load measurement should be repeated every 4-6 months if patient is clinically stable. If viral load returns to 0.3-0.5 log of pre-treatment levels, then the therapy is no longer working and should be changed.

b. CD4 count

  1. Initiation – within 2-4 weeks of starting treatment, CD4 count should be increased by at least 30 cells/mm3. If this is not achieved, then the therapy should be changed.
  2. Monitoring – CD4 counts should be obtained every 3-6 months during periods of clinical stability, and more frequently should symptomatic disease occurs. If CD4 count drops to baseline (or below 50% of increase from pre-treatment), then the therapy should be changed.

c. Anti-HIV Drug Resistance Testing

Anti-retroviral  drug resistance testing has become part and parcel of patient management in N. America and W. Europe. Many studies in treatment experienced patients have shown strong associations between the presence of drug resistance and failure of the antiretroviral treatment regimen to suppress HIV replication.

August 10th, 2011  |  Posted in Treatment of HIV  |  No Comments »

Antiviral Susceptibility Testing

Author: admin

Because of the increasing range of anti-HIV agents available, there is increasing pressure on the provision of antiviral susceptibility assays. There are two types of antiviral susceptibility assays: phenotypic and genotypic assays

Phenotypic assays define whether a particular strain of virus is sensitive or resistant to an antiviral agent by determining the concentration of the drug needed to inhibit the growth of virus in vitro. e.g. Plaque-reduction assay for HSV, plaque-reduction assay for HIV. However, phenotypic assays can only be used for viruses that can be cultivated. Moreover, in the case of HIV, plaque reduction assays may not be that appropriate since not all HIV strains produce plaques in cell culture.

In the case of genotypic assays, mutations that are associated with resistance are assayed for by molecular biology methods such as PCR and LCR.  However, these assays are tedious and are not suitable for a routine diagnostic laboratory. Moreover, he results of genotypic assays may prove very difficult to interpret since HIV  mutates at a furious pace, and it is also possible that resistant strains are present right at the beginning of infection.

Link to IAPAC article “Antiretroviral Drug Resistance and the Role of Resistance Monitoring”

March 28th, 2011  |  Posted in Diagnosis of HIV  |  No Comments »

CD4 Counts

Author: admin

HIV viral load – It appears that HIV viral load has the greatest prognostic value. HIV viral load in serum may be measured by assays which detect HIV-RNA e.g. RT-PCR, NASBA, or bDNA. HIV viral load has now been established as having good prognostic value, and in monitoring response to antiviral chemotherapy. Patients with a low viral load during the incubation period had a better prognosis than those with a high viral load. Patients whose viral load decreased significantly following the commencement of antiviral therapy had a better prognosis than those who did not respond.Among patients who responded to antiviral therapy, those who had a low pre-treatment viral load had a better prognosis than those who had a high pre-treatment viral load.

February 3rd, 2011  |  Posted in Diagnosis of HIV  |  No Comments »

Anti-Retroviral Agents

Author: admin

A. Nucleoside Reverse Transcriptase Inhibitor  

  1. Zidovudine (AZT)
  2. Didanosine (ddI)
  3. Zalcitabine (ddC)
  4. Emtricitabine (FTC)
  5. Lamivudine (3TC)
  6. Tenofovir (TDF)
  7. Stavudine (d4T)
  8. Abacavir (ABC)

B. Non-Nucleoside Reverse Transcriptase Inhibitor

  1. Nevirapine (NVP)
  2. Delaviridine (DLV)
  3. Efavirenz (EFV)

C. HIV Protease Inhibitors

  1. Tipranavi (TPV)
  2. Amprenavir (APV)
  3. Indinavir (IDV)
  4. Saquinavir (SQV)
  5. Ritonavir (RTV)
  6. Atazanavir (ATV)
  7. Fosamprenavir (FPV)
  8. Nelfinavir (NFV)

D. HIV Fusion Inhibitors

  1. Enfuvirtid (T-20)
  2. Maraviroc (MVC)

There are a number of combination preparations on the market e.g. CBV (AZT+3TC), TZV (AZT+3TC+ABC), TVD (FTC+TDF), Kaletra (Lopinavir/ritonavir). The use of combination preparations will reduce the number of tablets that need to be taken each time.

February 2nd, 2011  |  Posted in Treatment of HIV  |  No Comments »

HIV Viral Load

Author: admin

It appears that HIV viral load has the greatest prognostic value. HIV viral load in serum may be measured by assays which detect HIV-RNA e.g. RT-PCR, NASBA, or bDNA. HIV viral load has now been established as having good prognostic value, and in monitoring response to antiviral chemotherapy. Patients with a low viral load during the incubation period had a better prognosis than those with a high viral load. Patients whose viral load decreased significantly following the commencement of antiviral therapy had a better prognosis than those who did not respond.Among patients who responded to antiviral therapy, those who had a low pre-treatment viral load had a better prognosis than those who had a high pre-treatment viral load.

February 1st, 2011  |  Posted in Diagnosis of HIV  |  No Comments »

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